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High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing.

ABSTRACT: There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.

SUBMITTER: Jalali-Yazdi F 

PROVIDER: S-EPMC4834215 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing.

Jalali-Yazdi Farzad F   Lai Lan Huong LH   Takahashi Terry T TT   Roberts Richard W RW  

Angewandte Chemie (International ed. in English) 20160223 12

There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement. ...[more]

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