A label-free approach by infrared spectroscopic imaging for interrogating the biochemistry of diabetic nephropathy progression.

ABSTRACT: Routine histology, the current gold standard, involves staining for specific biomolecules. However, untapped biochemical information in tissue can be gathered using biochemical imaging. Infrared spectroscopy is an emerging modality that allows label-free chemical imaging to derive biochemical information (such as protein, lipids, DNA, collagen) from tissues. Here we employed this technology in order to better predict the development of diabetic nephropathy. Using human primary kidney biopsies or nephrectomies, we obtained tissue from 4 histologically normal kidneys, 4 histologically normal kidneys from diabetic subjects, and 5 kidneys with evidence of diabetic nephropathy. A biochemical signature of diabetic nephropathy was derived that enabled prediction of nephropathy based on the ratio

SUBMITTER: Varma VK 

PROVIDER: S-EPMC4834260 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature