Minimizing Postsampling Degradation of Peptides by a Thermal Benchtop Tissue Stabilization Method.

ABSTRACT: Enzymatic degradation is a major concern in peptide analysis. Postmortem metabolism in biological samples entails considerable risk for measurements misrepresentative of true in vivo concentrations. It is therefore vital to find reliable, reproducible, and easy-to-use procedures to inhibit enzymatic activity in fresh tissues before subjecting them to qualitative and quantitative analyses. The aim of this study was to test a benchtop thermal stabilization method to optimize measurement of endogenous opioids in brain tissue. Endogenous opioid peptides are generated from precursor proteins through multiple enzymatic steps that include conversion of one bioactive peptide to another, often with a different function. Ex vivo metabolism may, therefore, lead to erroneous functional interpretations

SUBMITTER: Segerstrom L 

PROVIDER: S-EPMC4834486 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature