T cell fate and clonality inference from single-cell transcriptomes.

ABSTRACT: We developed TraCeR, a computational method to reconstruct full-length, paired T cell receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links T cell specificity with functional response by revealing clonal relationships between cells alongside their transcriptional profiles. We found that T cell clonotypes in a mouse Salmonella infection model span early activated CD4(+) T cells as well as mature effector and memory cells.

SUBMITTER: Stubbington MJT 

PROVIDER: S-EPMC4835021 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature