Functional Dissection of an Alternatively Spliced Herpesvirus Gene by Splice Site Mutagenesis.

ABSTRACT:

Unlabelled

Herpesviruses have large and complex DNA genomes. The largest among the herpesviruses, those of the cytomegaloviruses, include over 170 genes. Although most herpesvirus gene products are expressed from unspliced transcripts, a substantial number of viral transcripts are spliced. Some viral transcripts are subject to alternative splicing, which leads to the expression of several proteins from a single gene. Functional analysis of individual proteins derived from an alternatively spliced gene is difficult, as deletion and nonsense mutagenesis, both common methods used in the generation of viral gene knockout mutants, affect several or all gene products at the same time. Here, we show that individual gene products of an alternatively spliced herpesvirus gene can be inactiva

SUBMITTER: Schommartz T 

PROVIDER: S-EPMC4836318 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature