M-Trap: Exosome-Based Capture of Tumor Cells as a New Technology in Peritoneal Metastasis.

ABSTRACT:

Background

Remodeling targeted tissues for reception of tumor cells metastasizing from primary lesions is a consequence of communication between the tumor and the environment that governs metastasis. This study describes a novel approach that aims to disrupt the process of metastasis by interfering with this intense dialogue.

Methods

Proteomics and adhesion assays identified exosomes purified from the ascitic fluid of ovarian cancer patients (n = 9) as intermediaries of tumor cell attachment. A novel tumor cell capture device was fabricated by embedding exosomes onto a 3D scaffold (metastatic trap [M-Trap]). Murine models of ovarian metastasis (n = 3 to 34 mice per group) were used to demonstrate the efficacy of M-Trap to capture metastatic cells disseminating in the periton

SUBMITTER: de la Fuente A 

PROVIDER: S-EPMC4836824 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature