Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection.

ABSTRACT:

Background

Dengue virus (DENV) and chikungunya virus (CHIKV) now co-circulate throughout tropical regions of the world, with billions of people living at risk of infection. The differentiation of these infections is important for epidemiologic surveillance as well as clinical care, though widely-used molecular diagnostics for DENV and CHIKV require the performance of two to four separate PCR reactions for detection.

Objectives

In the current study, we sought to develop and evaluate a single-reaction, multiplex real-time RT-PCR (rRT-PCR) for the detection and differentiation of DENV and CHIKV (the pan-DENV-CHIKV rRT-PCR).

Study design

From an alignment of all available CHIKV complete genome sequences in GenBank, a new CHIKV rRT-PCR was designed for use in multiplex wit

SUBMITTER: Waggoner JJ 

PROVIDER: S-EPMC4836994 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature