In silico analysis of regulatory and structural motifs of the ovine HSP90AA1 gene.

ABSTRACT: Gene promoters are essential regions of DNA where the transcriptional molecular machinery to produce RNA molecules is recruited. In this process, DNA epigenetic modifications can acquire a fundamental role in the regulation of gene expression. Recently, in a previous work of our group, functional features and DNA methylation involved in the ovine HSP90AA1 gene expression regulation have been observed. In this work, we report a combination of methylation analysis by bisulfite sequencing in several tissues and at different developmental stages together with in silico bioinformatic analysis of putative regulating factors in order to identify regulative mechanisms both at the promoter and gene body. Our results show a "hybrid structure" (TATA box + CpG island) of the ovine HSP90AA1 gene promot

SUBMITTER: Gonzalez C 

PROVIDER: S-EPMC4837184 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature