The effects of arginine glutamate, a promising excipient for protein formulation, on cell viability: Comparisons with NaCl.

ABSTRACT: The effects of an equimolar mixture of l-arginine and l-glutamate (Arg·Glu) on cell viability and cellular stress using in vitro cell culture systems are examined with reference to NaCl, in the context of monoclonal antibody formulation. Cells relevant to subcutaneous administration were selected: the human monocyte cell line THP-1, grown as a single cell suspension, and adherent human primary fibroblasts. For THP-1 cells, the mechanism of cell death caused by relatively high salt concentrations was investigated and effects on cell activation/stress assessed as a function of changes in membrane marker and cytokine (interleukin-8) expression. These studies demonstrated that Arg·Glu does not have any further detrimental effects on THP-1 viability in comparison to NaCl at equivalent osmolalit

SUBMITTER: Kheddo P 

PROVIDER: S-EPMC4837223 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature