Thermodynamics of Damaged DNA Binding and Catalysis by Human AP Endonuclease 1.

ABSTRACT: Apurinic/apyrimidinic (AP) endonucleases play an important role in DNA repair and initiation of AP site elimination. One of the most topical problems in the field of DNA repair is to understand the mechanism of the enzymatic process involving the human enzyme APE1 that provides recognition of AP sites and efficient cleavage of the 5'-phosphodiester bond. In this study, a thermodynamic analysis of the interaction between APE1 and a DNA substrate containing a stable AP site analog lacking the C1' hydroxyl group (F site) was performed. Based on stopped-flow kinetic data at different temperatures, the steps of DNA binding, catalysis, and DNA product release were characterized. The changes in the standard Gibbs energy, enthalpy, and entropy of sequential specific steps of the repair process wer

SUBMITTER: Miroshnikova AD 

PROVIDER: S-EPMC4837577 | biostudies-literature | 2016 Jan-Mar

REPOSITORIES: biostudies-literature