Relationship between Milk Microbiota, Bacterial Load, Macronutrients, and Human Cells during Lactation.

ABSTRACT: Human breast milk is considered the optimal nutrition for infants, providing essential nutrients and a broad range of bioactive compounds, as well as its own microbiota. However, the interaction among those components and the biological role of milk microorganisms is still uncovered. Thus, our aim was to identify the relationships between milk microbiota composition, bacterial load, macronutrients, and human cells during lactation. Bacterial load was estimated in milk samples from a total of 21 healthy mothers through lactation time by bacteria-specific qPCR targeted to the single-copy gene fusA. Milk microbiome composition and diversity was estimated by 16S-pyrosequencing and the structure of these bacteria in the fluid was studied by flow cytometry, qPCR, and microscopy. Fat, protein, la

SUBMITTER: Boix-Amoros A 

PROVIDER: S-EPMC4837678 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature