Development and characterization of novel 8-plex DiLeu isobaric labels for quantitative proteomics and peptidomics.

ABSTRACT:

Rationale

Relative quantification of proteins via their enzymatically digested peptide products determines disease biomarker candidate lists in discovery studies. Isobaric label-based strategies using TMT and iTRAQ allow for up to 10 samples to be multiplexed in one experiment, but their expense limits their use. The demand for cost-effective tagging reagents capable of multiplexing many samples led us to develop an 8-plex version of our isobaric labeling reagent, DiLeu.

Methods

The original 4-plex DiLeu reagent was extended to an 8-plex set by coupling isotopic variants of dimethylated leucine to an alanine balance group designed to offset the increasing mass of the label's reporter group. Tryptic peptides from a single protein digest, a protein mixture digest, and Saccharo

SUBMITTER: Frost DC 

PROVIDER: S-EPMC4837894 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature