Enhanced sequencing coverage with digital droplet multiple displacement amplification.

ABSTRACT: Sequencing small quantities of DNA is important for applications ranging from the assembly of uncultivable microbial genomes to the identification of cancer-associated mutations. To obtain sufficient quantities of DNA for sequencing, the small amount of starting material must be amplified significantly. However, existing methods often yield errors or non-uniform coverage, reducing sequencing data quality. Here, we describe digital droplet multiple displacement amplification, a method that enables massive amplification of low-input material while maintaining sequence accuracy and uniformity. The low-input material is compartmentalized as single molecules in millions of picoliter droplets. Because the molecules are isolated in compartments, they amplify to saturation without competing for re

SUBMITTER: Sidore AM 

PROVIDER: S-EPMC4838355 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature