Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions.

ABSTRACT: The identification of proteins that interact with small bioactive molecules is a critical but often difficult and time-consuming step in understanding cellular signaling pathways or molecular mechanisms of drug action. Numerous methods for identifying small molecule-interacting proteins have been developed and utilized, including affinity-based purification followed by mass spectrometry analysis, protein microarrays, phage display, and three-hybrid approaches. Although all these methods have been used successfully, there remains a need for additional techniques for analyzing small molecule-protein interactions. A promising method for identifying small molecule-protein interactions is affinity-based selection of yeast surface-displayed human proteome libraries. Large and diverse libraries d

SUBMITTER: Bidlingmaier S 

PROVIDER: S-EPMC4838597 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature