A simple method for plasma total vitamin C analysis suitable for routine clinical laboratory use.

ABSTRACT:

Background

In-hospital hypovitaminosis C is highly prevalent but almost completely unrecognized. Medical awareness of this potentially important disorder is hindered by the inability of most hospital laboratories to determine plasma vitamin C concentrations. The availability of a simple, reliable method for analyzing plasma vitamin C could increase opportunities for routine plasma vitamin C analysis in clinical medicine.

Methods

Plasma vitamin C can be analyzed by high performance liquid chromatography (HPLC) with electrochemical (EC) or ultraviolet (UV) light detection. We modified existing UV-HPLC methods for plasma total vitamin C analysis (the sum of ascorbic and dehydroascorbic acid) to develop a simple, constant-low-pH sample reduction procedure followed by isocratic r

SUBMITTER: Robitaille L 

PROVIDER: S-EPMC4839128 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature