Quantitative Microplate Assay for Real-Time Nuclease Kinetics.

ABSTRACT: Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.

SUBMITTER: Eriksson J 

PROVIDER: S-EPMC4839650 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature