Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq.

ABSTRACT: Despite the importance of the mammalian neocortex for complex cognitive processes, we still lack a comprehensive description of its cellular components. To improve the classification of neuronal cell types and the functional characterization of single neurons, we present Patch-seq, a method that combines whole-cell electrophysiological patch-clamp recordings, single-cell RNA-sequencing and morphological characterization. Following electrophysiological characterization, cell contents are aspirated through the patch-clamp pipette and prepared for RNA-sequencing. Using this approach, we generate electrophysiological and molecular profiles of 58 neocortical cells and show that gene expression patterns can be used to infer the morphological and physiological properties such as axonal arborizati

SUBMITTER: Cadwell CR 

PROVIDER: S-EPMC4840019 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature