HT-COMET: a novel automated approach for high throughput assessment of human sperm chromatin quality.

ABSTRACT:

Study question

Can we make the comet assay (single-cell gel electrophoresis) for human sperm a more accurate and informative high throughput assay?

Summary answer

We developed a standardized automated high throughput comet (HT-COMET) assay for human sperm that improves its accuracy and efficiency, and could be of prognostic value to patients in the fertility clinic.

What is known already

The comet assay involves the collection of data on sperm DNA damage at the level of the single cell, allowing the use of samples from severe oligozoospermic patients. However, this makes comet scoring a low throughput procedure that renders large cohort analyses tedious. Furthermore, the comet assay comes with an inherent vulnerability to variability. Our objective is to develop an au

SUBMITTER: Albert O 

PROVIDER: S-EPMC4840022 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature