Inhibition of Notch Signaling During Mouse Incisor Renewal Leads to Enamel Defects.

ABSTRACT: The continuously growing rodent incisor is an emerging model for the study of renewal of mineralized tissues by adult stem cells. Although the Bmp, Fgf, Shh, and Wnt pathways have been studied in this organ previously, relatively little is known about the role of Notch signaling during incisor renewal. Notch signaling components are expressed in enamel-forming ameloblasts and the underlying stratum intermedium (SI), which suggested distinct roles in incisor renewal and enamel mineralization. Here, we injected adult mice with inhibitory antibodies against several components of the Notch pathway. This blockade led to defects in the interaction between ameloblasts and the SI cells, which ultimately affected enamel formation. Furthermore, Notch signaling inhibition led to the downregulation of

SUBMITTER: Jheon AH 

PROVIDER: S-EPMC4840178 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature