Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams.

ABSTRACT:

Purpose

To investigate the variations in induction and repair of DNA damage along the proton path, after a previous report on the increasing biological effectiveness along clinically modulated 60-MeV proton beams.

Methods and materials

Human skin fibroblast (AG01522) cells were irradiated along a monoenergetic and a modulated spread-out Bragg peak (SOBP) proton beam used for treating ocular melanoma at the Douglas Cyclotron, Clatterbridge Centre for Oncology, Wirral, Liverpool, United Kingdom. The DNA damage response was studied using the 53BP1 foci formation assay. The linear energy transfer (LET) dependence was studied by irradiating the cells at depths corresponding to entrance, proximal, middle, and distal positions of SOBP and the entrance and peak position for the pris

SUBMITTER: Chaudhary P 

PROVIDER: S-EPMC4840231 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature