Ultra-deep and quantitative saliva proteome reveals dynamics of the oral microbiome.

ABSTRACT:

Background

The oral cavity is home to one of the most diverse microbial communities of the human body and a major entry portal for pathogens. Its homeostasis is maintained by saliva, which fulfills key functions including lubrication of food, pre-digestion, and bacterial defense. Consequently, disruptions in saliva secretion and changes in the oral microbiome contribute to conditions such as tooth decay and respiratory tract infections. Here we set out to quantitatively map the saliva proteome in great depth with a rapid and in-depth mass spectrometry-based proteomics workflow.

Methods

We used recent improvements in mass spectrometry (MS)-based proteomics to develop a rapid workflow for mapping the saliva proteome quantitatively and at great depth. Standard clinical cotton s

SUBMITTER: Grassl N 

PROVIDER: S-EPMC4841045 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature