Skeletal regeneration in the brittle star Amphiura filiformis.

ABSTRACT:

Background

Brittle stars regenerate their whole arms post-amputation. Amphiura filiformis can now be used for molecular characterization of arm regeneration due to the availability of transcriptomic data. Previous work showed that specific developmental transcription factors known to take part in echinoderm skeletogenesis are expressed during adult arm regeneration in A. filiformis; however, the process of skeleton formation remained poorly understood. Here, we present the results of an in-depth microscopic analysis of skeletal morphogenesis during regeneration, using calcein staining, EdU labeling and in situ hybridization.

Results

To better compare different samples, we propose a staging system for the early A. filiformis arm regeneration stages based on morphological land

SUBMITTER: Czarkwiani A 

PROVIDER: S-EPMC4841056 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature