Identification of OP354-like human rotavirus strains with subtype P[8]b in Ghanaian children with diarrhoea.

ABSTRACT:

Background

Rotaviruses with the P[8] genotype have been associated with majority of infections. Recent improvements in molecular diagnostics have delineated the P[8] genotype into P[8]a and P[8]b subtypes. P[8]a is the previously known P[8] genotype which is common whilst P[8]b subtype also known as OP354-like strain is genetically distinct, rarely detected and reported from a few countries. In a previous study, the P-types could not be determined for 80 RVA-positive samples by conventional RT-PCR genotyping methods with the recommended pool of P-genotype specific primers used in the WHO Regional Rotavirus Reference Laboratory in Ghana. The present study employed sequence-dependent cDNA amplification method to genotype previously non-typeable P-types.

Methods

Viral RNAs were

SUBMITTER: Damanka S 

PROVIDER: S-EPMC4841057 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature