Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures.

ABSTRACT: Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill-Lepomis macrochirus; crappie-Pomoxis annularis), at varied time intervals and storage temperatures, to assess the histologic quality of collected samples. A pooled sample of 36 mix sex individuals of healthy bluegill and crappie were euthanized, stored either at room temperature, refrigerated at 4 °C, or frozen at -20 °C, and then necropsied at 0, 4, 24, and 48 h intervals. Histologic specimens were evaluated by light microscopy. Data showed that immediate harvesting of fresh samples provides the best quality a

SUBMITTER: George J 

PROVIDER: S-EPMC4841231 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature