Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.

ABSTRACT: Female meiosis is a fundamental area of study in reproductive medicine, and the mouse oocyte model of in vitro maturation (IVM) is most widely used to study female meiosis. To investigate the probable role(s) of an unknown protein in female meiosis, the method traditionally used involves microinjecting a specific antibody into mouse oocytes. Recently, in studies on somatic cells, peptide nanoparticle-mediated antibody transfection has become a popular tool because of its high efficiency, low toxicity, good stability, and strong serum compatibility. However, untill now no researchers have tried using this technique on mouse oocytes because the zona pellucida surrounding the oocyte membrane (vitelline membrane) is usually thought or proved to be a tough barrier to macromolecules such as anti

SUBMITTER: Li R 

PROVIDER: S-EPMC4841238 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature