Autophagy inhibition enhances RAD001-induced cytotoxicity in human bladder cancer cells.

ABSTRACT:

Background

Mammalian target of rapamycin (mTOR), involved in PI3K/AKT/mTOR pathway, is known to play a central role in regulating the growth of cancer cells. The PI3K/AKT/mTOR pathway enhances tumor survival and proliferation through suppressing autophagy, which sustains energy homeostasis by collecting and recycling cellular components under stress conditions. Conversely, inhibitors of the mTOR pathway such as RAD001 induce autophagy, leading to promotion of tumor survival and limited antitumor efficacy. We thus hypothesized that the use of autophagy inhibitor in combination with mTOR inhibition improves the cytotoxicity of mTOR inhibitors in bladder cancer.

Materials and methods

The cytotoxicity of RT4, 5637, HT1376, and T24 human bladder cancer cells treated with RAD001 a

SUBMITTER: Lin JF 

PROVIDER: S-EPMC4841413 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature