Optimizing Mass Spectrometry Analyses: A Tailored Review on the Utility of Design of Experiments.

ABSTRACT: Mass spectrometry (MS) has emerged as a tool that can analyze nearly all classes of molecules, with its scope rapidly expanding in the areas of post-translational modifications, MS instrumentation, and many others. Yet integration of novel analyte preparatory and purification methods with existing or novel mass spectrometers can introduce new challenges for MS sensitivity. The mechanisms that govern detection by MS are particularly complex and interdependent, including ionization efficiency, ion suppression, and transmission. Performance of both off-line and MS methods can be optimized separately or, when appropriate, simultaneously through statistical designs, broadly referred to as "design of experiments" (DOE). The following review provides a tutorial-like guide into the selection of DO

SUBMITTER: Hecht ES 

PROVIDER: S-EPMC4841694 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature