A CRISPR/Cas9 system adapted for gene editing in marine algae.

ABSTRACT: Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

SUBMITTER: Nymark M 

PROVIDER: S-EPMC4842962 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature