Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells.

ABSTRACT: During cerebral development, many types of neurons are sequentially generated by self-renewing progenitor cells called apical progenitors (APs). Temporal changes in AP identity are thought to be responsible for neuronal diversity; however, the mechanisms underlying such changes remain largely unknown. Here we perform single-cell transcriptome analysis of individual progenitors at different developmental stages, and identify a subset of genes whose expression changes over time but is independent of differentiation status. Surprisingly, the pattern of changes in the expression of such temporal-axis genes in APs is unaffected by cell-cycle arrest. Consistent with this, transient cell-cycle arrest of APs in vivo does not prevent descendant neurons from acquiring their correct laminar fates. An

SUBMITTER: Okamoto M 

PROVIDER: S-EPMC4842982 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature