Hypoxia regulates global membrane protein endocytosis through caveolin-1 in cancer cells.

ABSTRACT: Hypoxia promotes tumour aggressiveness and resistance of cancers to oncological treatment. The identification of cancer cell internalizing antigens for drug targeting to the hypoxic tumour niche remains a challenge of high clinical relevance. Here we show that hypoxia down-regulates the surface proteome at the global level and, more specifically, membrane proteome internalization. We find that hypoxic down-regulation of constitutive endocytosis is HIF-independent, and involves caveolin-1-mediated inhibition of dynamin-dependent, membrane raft endocytosis. Caveolin-1 overexpression inhibits protein internalization, suggesting a general negative regulatory role of caveolin-1 in endocytosis. In contrast to this global inhibitory effect, we identify several proteins that can override caveolin-

SUBMITTER: Bourseau-Guilmain E 

PROVIDER: S-EPMC4842985 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature