Molecular Characterisation of Clinical Isolates of Chikungunya Virus: A Study from Tertiary Care Hospitals in Southern India.

ABSTRACT:

Introduction

Indian ocean islands and India have experienced massive severe Chikungunya outbreak from 2005 up till now and then Chikungunya became epidemic in India. The mutations that occurred in E1 gene were responsible for increased infectivity, virulence and host adaptability. It is important to find out the genotype and its probable evolvement and novel mutations in the E1 gene reported during 2006-2009 from the current isolates, which may affect the local protein structure.

Aim

To perform Molecular diagnosis and Molecular Characterisation of Chikungunya virus isolates.

Materials and methods

A total of 33 samples were included in the study. RNA was isolated from 33 serum samples and Real time PCR was carried out. Further, Nested PCR and E1 partial gene sequencing

SUBMITTER: Mudurangaplar B 

PROVIDER: S-EPMC4843258 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature