The Polymorphisms in LNK Gene Correlated to the Clinical Type of Myeloproliferative Neoplasms.

ABSTRACT:

Objective

LNK is an adapter protein negatively regulating the JAK/STAT cell signaling pathway. In this study, we observed the correlation between variation in LNK gene and the clinical type of myeloproliferative neoplasms (MPN).

Methods

A total of 285 MPN cases were recruited, including essential thrombocythemia (ET) 154 cases, polycythemia vera (PV) 76 cases, primary myelofibrosis (PMF) 19 cases, and chronic myeloid leukemia (CML) 36 cases. Ninety-three healthy individuals were used as normal controls. V617F mutation in JAK2 was identified by allele-specific PCR method, RT-PCR was used for the detection of BCR/ABL1 fusion gene, and mutations and variations in coding exons and their flanking sequences of LNK gene were examined by PCR-sequencing.

Results

Missense mutat

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4844169 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature