A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor.

ABSTRACT: Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential to understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some TFs also possess an oligomerization domain suspected to potentiate DNA binding but for which the genome-wide influence remains poorly understood. Here we focus on the LEAFY transcription factor, a master regulator of flower development in angiosperms. We have determined the crystal structure of its conserved amino-terminal domain, revealing an unanticipated Sterile Alpha Motif oligomerization domain. We show that this domain is essential to LEAFY floral function. Moreover, combined biochemical and genome-wide assays suggest that oligomerization is require

SUBMITTER: Sayou C 

PROVIDER: S-EPMC4844672 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature