Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.

ABSTRACT: Early diagnosis of fungal infection is critical for initiating antifungal therapy and reducing the high mortality rate in immunocompromised patients. In this study, we focused on rapid and sensitive identification of clinically important Candida species, utilizing the variability in the length of the ITS2 rRNA gene and fluorescent capillary electrophoresis (f-ITS2-PCR-CE). The method was developed and optimized on 29 various Candida reference strains from which 26 Candida species were clearly identified, while Candida guilliermondii, C. fermentati, and C. carpophila, which are closely related, could not be distinguished. The method was subsequently validated on 143 blinded monofungal clinical isolates (comprising 26 species) and was able to identify 88% of species unambiguously. This indic

SUBMITTER: Obrucova H 

PROVIDER: S-EPMC4844707 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature