Automated, high-throughput, in vivo analysis of visual function using the zebrafish.

ABSTRACT:

Background

Modern genomics has enabled the identification of an unprecedented number of genetic variants, which in many cases are extremely rare, associated with blinding disorders. A significant challenge will be determining the pathophysiology of each new variant. The Zebrafish is an excellent model for the study of inherited diseases of the eye. By 5 days post-fertilization (dpf), they have quantifiable behavioral responses to visual stimuli. However, visual behavior assays can take several hours to perform or can only be assessed one fish at a time.

Results

To increase the throughput for vision assays, we used the Viewpoint Zebrabox to automate the visual startle response and created software, Visual Interrogation of Zebrafish Manipulations (VIZN), to automate data analy

SUBMITTER: Scott CA 

PROVIDER: S-EPMC4844763 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature