Combining measurements to estimate properties and characterization extent of complex biochemical mixtures; applications to Heparan Sulfate.

ABSTRACT: Complex mixtures of molecular species, such as glycoproteins and glycosaminoglycans, have important biological and therapeutic functions. Characterization of these mixtures with analytical chemistry measurements is an important step when developing generic drugs such as biosimilars. Recent developments have focused on analytical methods and statistical approaches to test similarity between mixtures. The question of how much uncertainty on mixture composition is reduced by combining several measurements still remains mostly unexplored. Mathematical frameworks to combine measurements, estimate mixture properties, and quantify remaining uncertainty, i.e. a characterization extent, are introduced here. Constrained optimization and mathematical modeling are applied to a set of twenty-three expe

SUBMITTER: Pradines JR 

PROVIDER: S-EPMC4845011 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature