Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng.

ABSTRACT:

Background

The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level.

Methods

Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods.

SUBMITTER: Sun H 

PROVIDER: S-EPMC4845045 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature