Mammalian target of rapamycin controls glucose consumption and redox balance in human Sertoli cells.

ABSTRACT:

Objective

To study the role of mammalian target of rapamycin (mTOR) in the regulation of human Sertoli cell (hSC) metabolism, mitochondrial activity, and oxidative stress.

Design

Experimental study.

Setting

University research center and private assisted reproductive technology centers.

Patient(s)

Six men with anejaculation (psychological, vascular, neurologic) and conserved spermatogenesis.

Intervention(s)

Testicular biopsies were used from patients under treatment for recovery of male gametes. Primary hSCs cultures were established from each biopsy and divided into a control group and one treated with rapamycin, the inhibitor of mTOR, for 24 hours.

Main outcome measure(s)

Cytotoxicity of hSCs to rapamycin was evaluated by sulforhodamine B assay.

SUBMITTER: Jesus TT 

PROVIDER: S-EPMC4845725 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature