Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study.

ABSTRACT: Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the histopathological diagnosis of each stage of CC, and we evaluated mRNA cervical expression levels of IL-4, IL-6, IL-10, TGF-β1, TNF-α and IFN-γ across the histopathological diagnosis and specific bacterial clusters. We determined the cervical microbiota by high throughput sequencing of 16S rDNA amplicons and classified it in community state types (CST). Mean difference analyses between alpha-diversity and histopathological diagnosis were carried out, as well as a β-diversity analysis within the h

SUBMITTER: Audirac-Chalifour A 

PROVIDER: S-EPMC4846060 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature