A novel platelet lysate hydrogel for endothelial cell and mesenchymal stem cell-directed neovascularization.

ABSTRACT:

Unlabelled

Mesenchymal stem cells (MSC) hold promise in promoting vascular regeneration of ischemic tissue in conditions like critical limb ischemia of the leg. However, this approach has been limited in part by poor cell retention and survival after delivery. New biomaterials offer an opportunity to localize cells to the desired tissue after delivery, but also to improve cell survival after delivery. Here we characterize the mechanical and microstructural properties of a novel hydrogel composed of pooled human platelet lysate (PL) and test its ability to promote MSC angiogenic activity using clinically relevant in vitro and in vivo models. This PL hydrogel had comparable storage and loss modulus and behaved as a viscoelastic solid similar to fibrin hydrogels despite having 1/4-1/1

SUBMITTER: Robinson ST 

PROVIDER: S-EPMC4846562 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature