Differential utilization of binding loop flexibility in T cell receptor ligand selection and cross-reactivity.

ABSTRACT: Complementarity determining region (CDR) loop flexibility has been suggested to play an important role in the selection and binding of ligands by T cell receptors (TCRs) of the cellular immune system. However, questions remain regarding the role of loop motion in TCR binding, and crystallographic structures have raised questions about the extent to which generalizations can be made. Here we studied the flexibility of two structurally well characterized αβ TCRs, A6 and DMF5. We found that the two receptors utilize loop motion very differently in ligand binding and cross-reactivity. While the loops of A6 move rapidly in an uncorrelated fashion, those of DMF5 are substantially less mobile. Accordingly, the mechanisms of binding and cross-reactivity are very different between the two TCRs: whe

SUBMITTER: Ayres CM 

PROVIDER: S-EPMC4846865 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature