Image-based compound profiling reveals a dual inhibitor of tyrosine kinase and microtubule polymerization.

ABSTRACT: Small-molecule compounds are widely used as biological research tools and therapeutic drugs. Therefore, uncovering novel targets of these compounds should provide insights that are valuable in both basic and clinical studies. I developed a method for image-based compound profiling by quantitating the effects of compounds on signal transduction and vesicle trafficking of epidermal growth factor receptor (EGFR). Using six signal transduction molecules and two markers of vesicle trafficking, 570 image features were obtained and subjected to multivariate analysis. Fourteen compounds that affected EGFR or its pathways were classified into four clusters, based on their phenotypic features. Surprisingly, one EGFR inhibitor (CAS 879127-07-8) was classified into the same cluster as nocodazole, a mi

SUBMITTER: Tanabe K 

PROVIDER: S-EPMC4846875 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature