Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genome.

ABSTRACT: The Polycomb repressive complexes PRC1 and PRC2 maintain embryonic stem cell (ESC) pluripotency by silencing lineage-specifying developmental regulator genes. Emerging evidence suggests that Polycomb complexes act through controlling spatial genome organization. We show that PRC1 functions as a master regulator of mouse ESC genome architecture by organizing genes in three-dimensional interaction networks. The strongest spatial network is composed of the four Hox gene clusters and early developmental transcription factor genes, the majority of which contact poised enhancers. Removal of Polycomb repression leads to disruption of promoter-promoter contacts in the Hox gene network. In contrast, promoter-enhancer contacts are maintained in the absence of Polycomb repression, with accompanying w

SUBMITTER: Schoenfelder S 

PROVIDER: S-EPMC4847639 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature