Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL.

ABSTRACT: Type II nonribosomal peptide synthetases (NRPS) generate exotic amino acid derivatives that, combined with additional pathways, form many bioactive natural products. One family of type II NRPSs produce pyrrole moieties, which commonly arise from proline oxidation while tethered to a conserved, type II peptidyl carrier protein (PCP), as exemplified by PltL in the biosynthesis of pyoluteorin. We sought to understand the structural role of pyrrole PCPs in substrate and protein interactions through the study of pyrrole analogs tethered to PltL. Solution-phase NMR structural analysis revealed key interactions in residues of helix II and III with a bound pyrrole moiety. Conservation of these residues among PCPs in other pyrrole containing pathways suggests a conserved mechanism for formation, mo

SUBMITTER: Jaremko MJ 

PROVIDER: S-EPMC4847951 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature