A Laminated Microfluidic Device for Comprehensive Preclinical Testing in the Drug ADME Process.

ABSTRACT: New techniques are urgently needed to replace conventional long and costly pre-clinical testing in the new drug administration process. In this study, a laminated microfluidic device was fabricated to mimic the drug ADME response test in vivo. This proposed device was loaded and cultured with functional cells for drug response investigation and organ tissues that are involved in ADME testing. The drug was introduced from the top of the device and first absorbed by the Caco-2 cell layer, and then metabolized by the primary hepatocyte layer. It subsequently interacted with the MCF-7 cell layer, distributed in the lung, heart and fat tissues, and was finally eliminated through the dialysis membrane. Throughout this on-chip ADME process, the proposed device can be used as a reliable tool to si

SUBMITTER: An F 

PROVIDER: S-EPMC4848557 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature