Role of Increased n-acetylaspartate Levels in Cancer.

ABSTRACT:

Background

The clinical and biological effects of metabolic alterations in cancer are not fully understood.

Methods

In high-grade serous ovarian cancer (HGSOC) samples (n = 101), over 170 metabolites were profiled and compared with normal ovarian tissues (n = 15). To determine NAT8L gene expression across different cancer types, we analyzed the RNA expression of cancer types using RNASeqV2 data available from the open access The Cancer Genome Atlas (TCGA) website (http://www.cbioportal.org/public-portal/). Using NAT8L siRNA, molecular techniques and histological analysis, we determined cancer cell viability, proliferation, apoptosis, and tumor growth in in vitro and in vivo (n = 6-10 mice/group) settings. Data were analyzed with the Student's t test and Kaplan-Meier analysis

SUBMITTER: Zand B 

PROVIDER: S-EPMC4849357 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature