Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Muller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.

ABSTRACT:

Purpose

The purpose of this study was to investigate the effect of the antiviral drug ganciclovir (GCV) on Müller glia dedifferentiation and proliferation and the underlying cellular and molecular mechanisms in adult zebrafish.

Methods

A Tg(1016tuba1a:GFP) transgenic line was generated to identify injury-induced dedifferentiation of Müller glia. Mechanical retinal damage was induced by a needle-poke injury on the back of the eyes in adult zebrafish. Phosphate-buffered saline or GCV was injected into the vitreous of the eye at the time of injury or through the cornea. The GCV clearance rate from the eye was determined by a reversed-phase HPLC method. Green fluorescent protein (GFP) and bromodeoxyuridine (BrdU) immunofluorescence were used to determine the effect of GCV on ret

SUBMITTER: Zhang S 

PROVIDER: S-EPMC4849886 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature