Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.

ABSTRACT: Active Cdc42 is essential for the establishment of polarized growth. This GTPase is negatively regulated by the GTPase-activating proteins (GAPs), which are important for the spatial specificity of Cdc42 function. Rga4 is the only GAP described as negative regulator of fission yeast Cdc42. We report here that Rga6 is another fission yeast Cdc42 GAP which shares some functions with Rga4. Cells lacking Rga6 are viable but slightly shorter and broader than wild type, and cells lacking Rga6 and Rga4 simultaneously are rounded. In these cells, active Cdc42 is observed all around the membrane. These additive effects indicate that both GAPs collaborate in the spatial regulation of active Cdc42. Rga6 localizes to the plasma membrane forming clusters different from those formed by Rga4. A polybasic

SUBMITTER: Revilla-Guarinos MT 

PROVIDER: S-EPMC4850039 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature