Differential Roles of the NADPH-Oxidase 1 and 2 in Platelet Activation and Thrombosis.

ABSTRACT:

Objective

Reactive oxygen species (ROS) are known to regulate platelet activation; however, the mechanisms of ROS production during platelet activation remain unclear. Platelets express different isoforms of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) oxidases (NOXs). Here, we investigated the role of NOX1 and NOX2 in ROS generation and platelet activation using NOX1 and NOX2 knockout mice.

Approach and results

NOX1(-/Y) platelets showed selective defects in G-protein-coupled receptor-mediated platelet activation induced by thrombin and thromboxane A2 analog U46619, but were not affected in platelet activation induced by collagen-related peptide, a glycoprotein VI agonist. In contrast, NOX2(-/-) platelets showed potent inhibition of collagen-related peptide-induc

SUBMITTER: Delaney MK 

PROVIDER: S-EPMC4850088 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature