Molecular Dissection of Xyloglucan Recognition in a Prominent Human Gut Symbiont.

ABSTRACT:

Unlabelled

Polysaccharide utilization loci (PUL) within the genomes of resident human gut Bacteroidetes are central to the metabolism of the otherwise indigestible complex carbohydrates known as "dietary fiber." However, functional characterization of PUL lags significantly behind sequencing efforts, which limits physiological understanding of the human-bacterial symbiosis. In particular, the molecular basis of complex polysaccharide recognition, an essential prerequisite to hydrolysis by cell surface glycosidases and subsequent metabolism, is generally poorly understood. Here, we present the biochemical, structural, and reverse genetic characterization of two unique cell surface glycan-binding proteins (SGBPs) encoded by a xyloglucan utilization locus (XyGUL) from Bacteroides ovat

SUBMITTER: Tauzin AS 

PROVIDER: S-EPMC4850273 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature